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Scientific
Imaging
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the very best in Scientific Imaging Systems |
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The
University of Virginia
are using capture systems and SPCI
software from Compix to capture
and acquire high resolution fluorescence images of Xenopus.
A number of conventional epifluoprescence systems as well
as conventional confocal and
2-photon confocal techniques are employed.
SPCI was
used to control the confocal as well as provide the data sets
for the deconvolution software.
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Localization
of GFP-GAP-43 fusion
protein images in developing Xenopus embryos were acquired with
same objective lens using Nikon epi-fluorescence based two-photon
excitation microscopy (TPEM),
laser scanning confocal microscopy (LSCM; Nikon
PCM 2000 and Simple PCI software),
and wide-field microscopy using digital deconvolution (DDM).
The details of the images are
superior in TPEM. Generally, deterioration and less details
of information of GFP-GAP-43 proteins were observed in all sections
of the tissue in LSCM mode.
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The DDM system
is not as well suited for tissue imaging as demonstrated in
the figure (images were deconvolved with 15 iterations.
Images courtesy of Dr. Ammasi Periasamy.
For further information or reprints please contact us at
support@digitalpixel.co.uk
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